SEMINAR @ SISSA, SBP, dott. Christian Grunwald 21 feb. 2006

[ICTP info point]

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STATISTICAL AND BIOLOGICAL PHYSICS SEMINAR
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Dott. Christian Grunwald
(Ruhr-Universitaet Bochum, Physikalische Chemie)

 
Title:
Protein-surface interactions
 
Tue.   21 Feb. 2006  @ 12.00
 

SISSA - Basement floor - room D

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Abstract:
 
The surface adsorption behaviour of proteins and other biomolecules 
currently attracts a lot of attention. Many applications in 
life-sciences depend on surface based assay systems e.g. 
DNA-microarrays, blotting techniques, surface plasmon resonance, 
protein chips and biosensors in general. Despite the fact that the 
protein-surface interactions often play a key role they are still not 
completely understood yet. Therefore it is important to set-up simple 
test systems which allows to study protein adsorption experimentally.
 
The talk will focus on specific1 and unspecific2 protein adsorption to 
self-assembled monolayers (SAMs) prepared from different thiols. After 
a short introduction to the model systems protein adsorption data from 
various experimental techniques is discussed. In these studies 
fluorescence and atomic force microscopy (AFM), as well as surface 
plasmon resonance (SPR) are involved. Finally, the adsorption state of 
the adsorbed proteins will be addressed (protein activity).
 
 
1)       Christian Grunwald, Wolfgang Eck, Norbert Opitz, Jürgen 
Kuhlmann and Christof Wöll: Fabrication of 2D-protein arrays using 
biotinylated thiols: results from fluorescence microscopy and atomic 
force microscopy, Phys. Chem. Chem. Phys. 6, 4358-4362 (2004)
 
2)       Christian Grunwald, Jürgen Kuhlmann and Christof Wöll: In 
deuterated water the unspecific adsorption of proteins is significantly 
slowed down: Results of an SPR study using model organic surfaces, 
Langmuir 21, 9017-9019 (2005)
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